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Item #:
68232413
Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, Sterile, 500 mL
Item #:
68232413
SGD 75
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A versatile buffer commonly used in procedures for generating dendritic cells from monocytes and in animal embryo transfer.
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What Stands Out
Sterile Solution
Each 500 mL bottle of PBS-EDTA is sterilized to ensure no contamination, providing a reliable medium for biological and laboratory applications.
Enhanced Buffering
The phosphate buffer maintains a stable pH, crucial for preserving cell viability and function during experiments, making it superior to competing products.
Convenient Packaging
Offered in a ready-to-use 500 mL volume, this PBS-EDTA solution reduces preparation time in the lab, catering to both small and large-scale research needs.
Product Details
| Item Weight | 1.1 lbs (500 grams) |
Who Should Buy?
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Cell Culture
Ideal for maintaining pH levels and osmolality in cell culture applications, ensuring optimal cell viability and growth.
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Biological Sample Storage
Suitable for preserving biological samples, as EDTA chelates metal ions, preventing degradation and maintaining sample integrity.
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Molecular Biology Applications
Commonly used in buffer solutions for DNA extraction and manipulation, providing a stable environment for enzymatic reactions.
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High-Volume Uses
Not suitable for large-scale applications where bulk solutions are required, as it comes in a 500 mL bottle.
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Non-Microbial Use
May not be appropriate for applications requiring growth promotion of specific microorganisms that require different buffers.
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Chemical Sensitivity
Not ideal for experiments involving significant chemical interactions that may affect EDTA functionality and experimental outcomes.
Product Description
Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, Sterile, 500 mL
About This Item
Looking for a high-quality solution for your buffer preparation needs? Look no further than our Phosphate Buffered Saline EDTA. This sterile solution is perfect for procedures such as generating dendritic cells from monocytes and animal embryo transfers. Our 1 X PBS-EDTA solution is perfect for use in cell culture and tissue culture procedures. With EDTA included in the buffered saline solution, you can rest assured that your research is in good hands. Our PBS-EDTA solution is also affordably priced, making it a top choice for researchers on a budget.
Plus, with our bulk PBS-EDTA solution available for sale, you can stock up and save even more. Don't settle for less when it comes to your research. Choose the best with our high-quality Phosphate Buffered Saline EDTA solution. Order online today and experience the difference for yourself!.
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Customer Questions & Answers
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Question:
What is Phosphate Buffered Saline EDTA used for?
Answer: Phosphate Buffered Saline EDTA, commonly known as PBS-EDTA, serves multiple purposes in biological laboratories. It is primarily used for cell culture applications, providing a stable environment for cells. The phosphate buffer maintains physiological pH while EDTA acts as a chelating agent, sequestering divalent cations that can interfere with enzymatic reactions. This makes PBS-EDTA ideal for processes like cell washing, immunostaining, and tissue preservation, ensuring cellular integrity during experiments. -
Question:
Is PBS-EDTA sterile, and why is it important?
Answer: Yes, the PBS-EDTA solution is sterilized during production, which is critical for maintaining cell viability in laboratory settings. Sterility prevents microbial contamination that could compromise experimental results, particularly in cell culture, where bacteria or fungi could alter growth conditions and outcomes. By using sterile PBS-EDTA, you ensure that your cells or samples are handled in a controlled environment, reducing the risk of unwanted variables in your research. -
Question:
How should Phosphate Buffered Saline EDTA be stored?
Answer: Phosphate Buffered Saline EDTA should be stored at room temperature, away from direct sunlight and extreme temperatures. Proper storage is essential to maintain its efficacy and sterility. Ensure that the bottle is tightly sealed and check for expiration dates before usage. Storing it correctly ensures that the PBS-EDTA will remain suitable for cellular and biochemical applications, enabling reliable results in your experiments. -
Question:
Can Phosphate Buffered Saline EDTA be used in DNA extraction?
Answer: Yes, PBS-EDTA is frequently utilized in DNA extraction protocols. The EDTA component chelates magnesium ions, inhibiting enzymes like DNases that may degrade DNA. This action preserves the integrity of the DNA during isolation processes. Researchers often choose PBS-EDTA when working with sensitive samples, ensuring a higher purity of extracted DNA for downstream applications, including cloning and sequencing. -
Question:
What is the importance of using PBS-EDTA in flow cytometry?
Answer: PBS-EDTA is essential in flow cytometry for maintaining cell integrity and enabling accurate analysis. The EDTA helps to prevent cell aggregation by chelating calcium ions, which are essential for cell adhesion. By using PBS-EDTA, researchers can obtain single-cell suspensions that facilitate precise measurements of cell characteristics, such as size and granularity, enhancing the reliability of flow cytometry results. -
Question:
Can I use PBS-EDTA on sensitive cell types?
Answer: PBS-EDTA is suitable for many sensitive cell types, including primary cells and stem cells, due to its gentle composition. It maintains a stable pH and minimizes stress on cells, which is vital for preserving their functionality. Researchers often prefer PBS-EDTA for washing or resuspending sensitive cells to minimize damage while ensuring a prompt and effective cell handling process. -
Question:
What are the components of Phosphate Buffered Saline EDTA?
Answer: Phosphate Buffered Saline EDTA consists of two primary components: phosphate buffer and ethylenediaminetetraacetic acid (EDTA). The phosphate buffer comprises sodium phosphate and potassium phosphate, enabling the solution to maintain a neutral pH. The EDTA acts as a chelating agent, binding to metal ions that could be detrimental to biological reactions. This formulation makes PBS-EDTA versatile for various laboratory applications, including cell maintenance and biochemical assays. -
Question:
Is there any special preparation needed before using PBS-EDTA?
Answer: Typically, PBS-EDTA is ready to use straight from the bottle without additional preparation. However, it is advisable to gently mix the solution if there is any visible sediment. Before application, especially in sensitive experiments, checking the sterility of the liquid is crucial to avoid introducing contaminants. Proper handling guidelines ensure optimal results in cell cultures and biochemical assays. -
Question:
Where can I buy Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, Sterile, 500 mL?
Answer: You can buy Phosphate Buffered Saline EDTA, 1 X PBS-EDTA, Sterile, 500 mL from Ubuy in Singapore. Ubuy provides a reliable platform for purchasing laboratory supplies and ensures that you have access to high-quality products for your scientific needs.
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SGD 75
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